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  • Featured Product
  • KD/KO Validated

RHOA Recombinant antibody, PBS Only

RHOA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240880E5

Cat no : 82760-2-PBS

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOA Recombinant antibody (82760-2-RR)

    WB analysis using 82760-2-RR (same clone as 82760-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RHOA Recombinant antibody (82760-2-RR)

    WB analysis of HEK-293 using 82760-2-RR (same clone as 82760-2-PBS)

    WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOA Recombinant antibody (82760-2-RR)

    IF Staining of HeLa using 82760-2-RR (same clone as 82760-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

Product Information

82760-2-PBS targets RHOA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RHOA fusion protein Ag1141
Full Name ras homolog gene family, member A
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.