RHOA Monoclonal antibody, PBS Only

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  WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RHOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66733-1-PBS

Clone No.1B3D7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

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Product Information

66733-1-PBS targets RHOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHOA fusion protein Ag1141
• Full Name: ras homolog gene family, member A
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC005976
• Gene Symbol: RHOA
• Gene ID (NCBI): 387
• RRID: AB_2882083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61586
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.