RHAG Monoclonal antibody

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  WB analysis of human placenta using 67714-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67714-1-Ig (RHAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67714-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human placenta using 67714-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human spleen using 67714-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 67714-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 67714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of human placenta using 67714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RHAG Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67714-1-Ig

Clone No.1C1C4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Ammonium transporter Rh type A, CD241, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type A glycoprotein, RH2, Rh50, RH50A, Rh50GP, RHAG, SLC42A1

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67714-1-Ig targets RHAG in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHAG fusion protein Ag30130
• Full Name: Rh-associated glycoprotein
• Calculated Molecular Weight: 409 aa, 44 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC012605
• Gene Symbol: RHAG
• Gene ID (NCBI): 6005
• RRID: AB_2882904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q02094
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.