RGS8 Polyclonal antibody

RGS8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27394-1-AP

Synonyms

Regulator of G-protein signaling 8

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Validation Data Gallery

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  WB analysis using 27394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27394-1-AP (RGS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27394-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27394-1-AP (RGS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 27394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 27394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 27394-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 27394-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27394-1-AP targets RGS8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS8 fusion protein Ag26535
• Full Name: regulator of G-protein signaling 8
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC069718
• Gene Symbol: RGS8
• Gene ID (NCBI): 85397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.