RGS7 Polyclonal antibody

RGS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14056-1-AP

Synonyms

Regulator of G-protein signaling 7, regulator of G protein signaling 7

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 14056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14056-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14056-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14056-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 14056-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14056-1-AP (RGS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14056-1-AP targets RGS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS7 fusion protein Ag4982
• Full Name: regulator of G-protein signaling 7
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC022009
• Gene Symbol: RGS7
• Gene ID (NCBI): 6000
• RRID: AB_2238316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.