RGS4 Monoclonal antibody

RGS4 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E2E2

Cat no : 66441-1-Ig

Synonyms

RGP4, RGS4, SCZD9

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Validation Data Gallery

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  WB analysis of Neuro-2a using 66441-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66441-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66441-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66441-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66441-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66441-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, C6 cells, Raji cells, SH-SY5Y cells, U-251 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66441-1-Ig targets RGS4 in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RGS4 fusion protein Ag6029
• Full Name: regulator of G-protein signaling 4
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC051869
• Gene Symbol: RGS4
• Gene ID (NCBI): 5999
• RRID: AB_2881811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publications

Species	Application	Title
mouse	IHC	Hippocampus; NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.; Authors - Yan-Cong Wang