RGS4 Polyclonal antibody

• Featured Product
• KD/KO Validated

RGS4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14530-1-AP

Synonyms

RGP4, Regulator of G-protein signaling 4, regulator of G protein signaling 4

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Validation Data Gallery

• 

  WB analysis of mouse cerebellum using 14530-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14530-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 14530-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14530-1-AP

  IP result of anti-RGS4 (IP:14530-1-AP, 4ug; Detection:14530-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14530-1-AP (RGS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14530-1-AP (RGS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14530-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RGS4 antibody (14530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse brain tissue, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung tissue, mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

14530-1-AP targets RGS4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS4 fusion protein Ag6029
• Full Name: regulator of G-protein signaling 4
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC051869
• Gene Symbol: RGS4
• Gene ID (NCBI): 5999
• RRID: AB_2269443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publications

Species	Application	Title
human,mouse	IHC	J Allergy Clin Immunol; RGS4 promotes allergen- and aspirin-associated airway hyperresponsiveness by inhibiting PGE2 biosynthesis.; Authors - Gordon S Wong ; KO Validated
mouse	WB	Arterioscler Thromb Vasc Biol; HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.; Authors - Shanshan Liu
human	WB,IHC	Oncotarget; Regulator of G protein signaling 4 inhibits human melanoma cells proliferation and invasion through the PI3K/AKT signaling pathway.; Authors - Xiaotong Xue
human	WB,IHC,IF	Onco Targets Ther; RGS4 Regulates Proliferation And Apoptosis Of NSCLC Cells Via microRNA-16 And Brain-Derived Neurotrophic Factor.; Authors - Zheng He
human	WB	Arch Gerontol Geriatr; LINC00370 modulates miR-222-3p-RGS4 axis to protect against osteoporosis progression.; Authors - Lintao Li
human	WB	Redox Biol; Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes; Authors - Yulong Zhang