RGS12 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85057-2-RR (same clone as 85057-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85057-2-RR (RGS12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01790-2

  Sandwich ELISA standard curve of MP01790-2, Human RGS12 Recombinant Matched Antibody Pair - PBS only. 85057-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30731. 85057-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01790-2

  The mean RGS12 concentration was determined to be 18,450.2 pg/mL in A431 cell extract based on a 1.3 mg/mL extract load, 6,327.2 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 42,390.5 pg/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

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  IF Staining of PC-3 using 85057-2-RR (same clone as 85057-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using RGS12 antibody (85057-2-RR, Clone: 242530G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01790-2

  Cytometric bead array standard curve of MP01790-2, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-3-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

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  Cytometric bead array standard curve of MP01790-1

  Cytometric bead array standard curve of MP01790-1, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-1-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 85057-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85057-2-RR against Human RGS12 were performed. The affinity constant is 0.611 nM.

RGS12 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85057-2-PBS

Clone No.242530G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242530G11

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Product Information

85057-2-PBS targets RGS12 as part of a matched antibody pair:

MP01790-1: 85057-1-PBS capture and 85057-2-PBS detection (validated in Cytometric bead array)

MP01790-2: 85057-3-PBS capture and 85057-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RGS12 fusion protein Ag30731
• Full Name: regulator of G-protein signaling 12
• Calculated Molecular Weight: 156 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_198229
• Gene Symbol: RGS12
• Gene ID (NCBI): 6002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14924
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.