RGL3 Polyclonal antibody

RGL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26626-1-AP

Synonyms

RalGDS like 3

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Validation Data Gallery

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  WB analysis using 26626-1-AP

  HepG2 cells, RT-4 cells, Caco-2 cells lysates were subjected to SDS PAGE followed by western blot with 26626-1-AP (RGL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RT-4 cells, HepG2 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26626-1-AP targets RGL3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGL3 fusion protein Ag24329
• Full Name: ral guanine nucleotide dissociation stimulator-like 3
• Calculated Molecular Weight: 710 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC101756
• Gene Symbol: RGL3
• Gene ID (NCBI): 57139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).