RFX4 Polyclonal antibody

RFX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13971-1-AP

Synonyms

NYD SP10, Regulatory factor X 4, RFX4, Transcription factor RFX4

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Validation Data Gallery

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  WB analysis using 13971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13971-1-AP (RFX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13971-1-AP targets RFX4 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFX4 fusion protein Ag5004
• Full Name: regulatory factor X, 4 (influences HLA class II expression)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC030644
• Gene Symbol: RFX4
• Gene ID (NCBI): 5992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFX4, also named as NYD-SP10, contains one RFX-type winged-helix DNA-binding domain and belongs to the RFX family. It may activate transcription by interacting directly with the X-box The protein is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This is a rabbit polyclonal antibody raised against the C terminus of human RFX4.