ChromoTek RFP VHH, recombinant binding protein

Alpaca anti-RFP VHH, purified recombinant binding protein

Specificity

RFP and RFP derivates

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Publications

6

Cat no : rt

Synonyms

RFP-Binding-Protein, RFP-Nanobody, and RFP-single domain antibody (sdAb), mCherry-Binding-Protein, mCherry-Nanobody, and mChery single domain antibody (sdAb)

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Validation Data Gallery

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  Structure of RFP-VHH.

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Product Information

Alpaca anti-RFP VHH, purified recombinant binding protein

• Applications: Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
• Specificity/Target: mCherry, mRFP, mRFPRuby, TagRFP, mKate2, mPlum, mApple, DsRed, tdTomato
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 5 nM
• Molecular Weight: 14.9 kDa
• RRID: AB_2631365
• Storage Buffer: 25mM TAPS pH 8.5, 137 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.
• Background: Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.; The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). ; When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.; This Nanobody is an alpaca recombinant VHH raised against RFP, reactive to a variety of RFP derivates, including mCherry, mRFP, mRFPRuby, TagRFP, mKate2, mPlum, mApple, DsRed, and tdTomato.

Publications

Application	Title
	Nat Methods; Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.; Authors - Yongdeng Zhang
	Plant J; BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidopsis.; Authors - Rosanna Petrella
IF	ACS Chem Biol; A simple method for GFP- and RFP-based dual color single-molecule localization microscopy.; Authors - Evgenia Platonova
IF	Methods Appl Fluoresc; Dual color single particle tracking via nanobodies.; Authors - David Albrecht
	Cell Chem Biol; The cyclimids: Degron-inspired cereblon binders for targeted protein degradation; Authors - Saki Ichikawa
IF,WB	J Vasc Res; Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis; Authors - Wei Chang