ChromoTek RFP Monoclonal antibody (5F8)

Rat monoclonal antibody [5F8] to Red Fluorescent Proteins

Host/IsoType

Rat/IgG2a

Specificity

dsRed, mRFP, mCherry, mPlum, mRFPruby, mScarlet, tdTomato

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

5F8

Publications

1

Cat no : 5f8-2

Synonyms

RFP, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  HeLa cells transiently transfected with MannosidaseII-tdTomato (Golgi). Primary antibody: [5F8] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 647 Fixation: 3.7 % PFA Scale bar: 10 µm

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Product Information

Rat monoclonal antibody [5F8] to Red Fluorescent Proteins

• Description: Rat monoclonal antibody [5F8] to Red Fluorescent Proteins
• Applications: IF, ELISA
• Specificity/Target: Tested on DsRed, mRFP, mCherry, mPlum, mRFPruby, mScarlet, tdTomato, mKate2, mOrange.
• Recommended Dilution: IF: recommended starting dilution 1:1,000; A titration from 1:200 up to 1:2,000 is recommended.
• Host/IsoType: Rat/IgG2a
• Immunogen: UniProtKB: Q9U6Y8
• Type: Primary Antibody
• Conjugate: Unconjugated
• Clonality: Monoclonal
• Clone No.: 5F8
• RRID: AB_2336064
• Purity: Protein A or G purified
• Form: Buffered aqueous solution
• Storage Buffer: PBS, 50% glycerol, preservative: 0.09% sodium azide.
• Storage Condition: Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.
• Size: 20 μL; 150 μL

Publications

Application	Title
IF	Nat Methods; An optogenetic method for the controlled release of single molecules; Authors - Purba Kashyap