ChromoTek RFP-Booster ATTO594

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

ATTO 594

Host

Alpaca

Publications

41

Cat no : rba594

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.

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Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

• Description: The RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Considerably higher tissue penetration rates

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Less than 2 nm epitope-label displacement minimizes linkage error

  • Monovalent VHHs do not cluster their epitopes

  • Validation: structure and function characterized

  • Consistent and reliable performance due to recombinant production

• Applications: IF, IHC, ICC
• Host: Alpaca
• Specificity/Target: mCherry, mPlum, mRFP, mRFPruby, mKate2; For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
• Conjugate: ATTO 594
• Physical State: Liquid
• Suggested Dilution: IF/ICC/IHC: 1:200
• Type: Nanobody
• Class: Recombinant
• RRID: AB_2631390
• Affinity (KD): Dissociation constant K_D of 5nM
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.
• Note: This product is for research use only, not for diagnostic or therapeutic use

Publications

Application	Title
IF	Science; SIGNAL TRANSDUCTION. Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling.; Authors - Yong Zhou
IF	Cell; Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.; Authors - Sahana Holla
IF	Cell; Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body.; Authors - Chenchen Pan
IF	Nature; Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.; Authors - Marina Vietri
IF	Nat Neurosci; Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections.; Authors - Ruiyao Cai
IF	Nat Cell Biol; Drosophila patterning is established by differential association of mRNAs with P bodies.; Authors - Timothy T Weil