RFC2 Polyclonal antibody

RFC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 10410-1-AP

Synonyms

RF C 40 kDa subunit, Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit

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Validation Data Gallery

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10410-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10410-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10410-1-AP

  IP result of anti-RFC2 (IP:10410-1-AP, 4ug; Detection:10410-1-AP 1:500) with HeLa cells lysate 3000 ug.

• 

  IF Staining of HeLa using 10410-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10410-1-AP (RFC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

10410-1-AP targets RFC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFC2 fusion protein Ag0663
• Full Name: replication factor C (activator 1) 2, 40kDa
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC002813
• Gene Symbol: RFC2
• Gene ID (NCBI): 5982
• RRID: AB_2285035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

Publications

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E
mouse	WB	Proc Natl Acad Sci U S A; Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.; Authors - Chiang Po-Min PM
human	WB	Sci Rep; RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.; Authors - Xu Zhao
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lei (Verified Customer) (11-29-2018)

This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2

RFC2 Antibody Western Blot, validation (1:500 dilution) in HepG2 (Cat no:10410-1-AP)