RFC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RFC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19159-1-AP

Synonyms

A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA binding protein PO GA, MHCBFB, PO GA, RECC1, Replication factor C subunit 1, RF C 140 kDa subunit, RFC, RFC1, RFC140

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Validation Data Gallery

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  WB analysis of Jurkat using 19159-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19159-1-AP (RFC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19159-1-AP

  WB result of RFC1 antibody (19159-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFC1 transfected HeLa cells.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19159-1-AP is RFC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFC1 fusion protein Ag13585
• Full Name: replication factor C (activator 1) 1, 145kDa
• Calculated Molecular Weight: 128 kDa
• Observed Molecular Weight: 128-150 kDa
• GenBank Accession Number: BC051751
• Gene Symbol: RFC1
• Gene ID (NCBI): 5981
• RRID: AB_2878574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.