REST Recombinant antibody

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  WB analysis using 84846-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84846-4-RR (REST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84846-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody (84846-4-RR, Clone: 241789D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

REST Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84846-4-RR

Clone No.241789D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

XBR, X2 box repressor, NRSF, 241789D3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, BxPC-3 cells, SW 1990 cells, L02 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84846-4-RR targets REST in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: REST fusion protein Ag17707
• Full Name: RE1-silencing transcription factor
• Calculated Molecular Weight: 1097 aa, 122 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC132859
• Gene Symbol: REST
• Gene ID (NCBI): 5978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13127
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822).