REPS2 Recombinant antibody

REPS2 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241136D3

Cat no : 84016-4-RR

Synonyms

REPS 2, RalBP1-interacting protein 2, RalBP1-associated Eps domain-containing protein 2, RalBP1 interacting protein 2, 241136D3

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Validation Data Gallery

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  IF Staining of LNCaP using 84016-4-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using REPS2 antibody (84016-4-RR, Clone: 241136D3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84016-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84016-4-RR against Human REPS2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84016-4-RR is REPS2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: REPS2 fusion protein Ag33764
• Full Name: RALBP1 associated Eps domain containing 2
• Calculated Molecular Weight: 72KD
• GenBank Accession Number: NM_004726
• Gene Symbol: REPS2
• Gene ID (NCBI): 9185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. There are 4 isoforms of the protein with two short (58 kDa) isoforms comprising 521 or 522 amino acid residues and two long (78 kDa) ones of 659 or 660 residues.