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  • KD/KO Validated

RENALASE Polyclonal antibody

RENALASE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 15003-1-AP

Synonyms

C10orf59, Monoamine oxidase-C, Monoamine oxidase C, MAO-C, MAO C



Tested Applications

Positive WB detected inCaco-2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15003-1-AP targets RENALASE in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RENALASE fusion protein Ag13061
Full Name chromosome 10 open reading frame 59
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC005364
Gene Symbol RENALASE
Gene ID (NCBI) 55328
RRIDAB_1557298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

Protocols

Product Specific Protocols
WB protocol for RENALASE antibody 15003-1-APDownload protocol
IHC protocol for RENALASE antibody 15003-1-APDownload protocol
IF protocol for RENALASE antibody 15003-1-APDownload protocol
IP protocol for RENALASE antibody 15003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB,IHC

Circulation

Catecholamines regulate the activity, secretion, and synthesis of renalase.

Authors - Li Guoyong G
human,ratWB,IHC

J Clin Invest

Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure.

Authors - Xu Jianchao J
mouseWB

Kidney Int

Renalase deficiency aggravates ischemic myocardial damage.

Authors - Wu Yanling Y
  • KO Validated
ratWB,IF

Front Cardiovasc Med

Aerobic Exercise Training Improves Renal Injury in Spontaneously Hypertensive Rats by Increasing Renalase Expression in Medulla.

Authors - Minghao Luo
humanWB,IF

Life Sci

Renalase is localized to the small intestine crypt and expressed upon the activation of NF-κB p65 in mice model of fasting-induced oxidative stress.

Authors - Kai Aoki
humanWB

Exp Biol Med (Maywood)

Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients.

Authors - Janete Quelhas-Santos