REN Polyclonal antibody

REN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29330-1-AP

Synonyms

Angiotensinogenase, FLJ10761, REN, renin

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Validation Data Gallery

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  WB analysis using 29330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29330-1-AP (REN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COS-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29330-1-AP targets REN in WB, ELISA applications and shows reactivity with Human, monkey samples.

• Tested Reactivity: Human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REN fusion protein Ag31071
• Full Name: renin
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC047752
• Gene Symbol: REN
• Gene ID (NCBI): 5972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Renin is an aspartic protease that consists of 2 homologous lobes, produced as pre-prorenin protein and transferred into the cisterns of the endoplasmic reticulum. Renin is mainly produced and released into circulation by the so-called juxtaglomerular epithelioid cells, located in the walls of renal afferent arterioles at the entrance of the glomerular capillary network (PMID: 16525949).REN has 2 isoforms produced by alternative splicing with a molecular weight of 45-47kDa.