RELL1 Polyclonal antibody

RELL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29823-1-AP

Synonyms

FLJ21778, RELL1, RELT like 1, RELT like protein 1

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 29823-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 29823-1-AP (RELL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat skin using 29823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 29823-1-AP (RELL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 29823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 29823-1-AP (RELL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: rat skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29823-1-AP targets RELL1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RELL1 fusion protein Ag31475
• Full Name: RELT-like 1
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC039540
• Gene Symbol: RELL1
• Gene ID (NCBI): 768211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELT-like protein 1 (RELL1) is a member of the tumor necrosis factor (TNF) receptor family and belongs to the receptor expressed in the lymphoid tissues (RELT) family. RELL1 activates the pro-inflammatory pathway by binding to TNF receptor-related factor 1 (TRAF1). Further, RELT family members activate p38 and induce apoptosis by a mechanism distinct from that of TNF receptors (PMID: 32090861). The molecular weight of RELL1 is 29 kDa.