REG4 Polyclonal antibody

REG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 12268-1-AP

Synonyms

GISP, REG 4, REG IV, REG like protein, REG4, RELP

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Validation Data Gallery

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  WB analysis of SGC-7901 using 12268-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12268-1-AP (REG4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12268-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12268-1-AP (REG4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12268-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12268-1-AP (REG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 12268-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12268-1-AP (REG4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, mouse liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

12268-1-AP targets REG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REG4 fusion protein Ag2934
• Full Name: regenerating islet-derived family, member 4
• Calculated Molecular Weight: 158 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC017089
• Gene Symbol: REG4
• Gene ID (NCBI): 83998
• RRID: AB_2178702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	Cancer Genomics Proteomics; Regulation of REG4 Expression and Prediction of 5-Fluorouracil Sensitivity by CDX2 in Ovarian Mucinous Carcinoma.; Authors - Iemasa Koh
human	IHC	J Surg Oncol; Overexpression of REG4 confers an independent negative prognosticator in rectal cancers receiving concurrent chemoradiotherapy.; Authors - Hong-Lin He
human	IHC	Signal Transduct Target Ther; Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis.; Authors - Xiaobo Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other