REG Recombinant antibody

REG Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241617H2

Cat no : 84287-3-RR

Synonyms

REG1 alpha, REG1A, Pancreatic stone protein, P19, Lithostathine-1-alpha

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Validation Data Gallery

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  WB analysis using 84287-3-RR

  mouse stomach tissue, mouse pancreas tissue, rat pancreas tissue,

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  IHC staining of mouse pancreas using 84287-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84287-3-RR (REG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84287-3-RR is REG Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: REG fusion protein Ag8640
• Full Name: regenerating islet-derived 1 alpha
• Calculated Molecular Weight: 166 aa, 19 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC005350
• Gene Symbol: REG
• Gene ID (NCBI): 5967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. It's reported that REG1A was significantly upregulated in tumor specimens and adenoma as compared to normal colorectal tissue. (PMID: 34698109)