REG Polyclonal antibody

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  WB analysis using 15850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15850-1-AP (REG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 15850-1-AP (REG antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15850-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

REG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15850-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

REG1 alpha, REG1A, Pancreatic thread protein, Pancreatic stone protein, P19

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human stomach cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15850-1-AP targets REG in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REG fusion protein Ag8640
• Full Name: regenerating islet-derived 1 alpha
• Calculated Molecular Weight: 166 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC005350
• Gene Symbol: REG
• Gene ID (NCBI): 5967
• RRID: AB_2878192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05451
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; Protective and anti-inflammatory role of REG1A in inflammatory bowel disease induced by JAK/STAT3 signaling axis.; Authors - Hongli Mao
human,mouse	WB,IF	Ann N Y Acad Sci; REG1A protects retinal photoreceptors from blue light damage; Authors - Ze-Hua Xu