REEP4 Polyclonal antibody

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  WB analysis of mouse testis using 26650-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26650-1-AP (REEP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 26650-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26650-1-AP (REEP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 26650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26650-1-AP (REEP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 26650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26650-1-AP (REEP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 26650-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using REEP4 antibody (26650-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

REEP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26650-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Receptor expression-enhancing protein 4, PP432, C8orf20

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26650-1-AP targets REEP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REEP4 fusion protein Ag24569
• Full Name: receptor accessory protein 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC013048
• Gene Symbol: REEP4
• Gene ID (NCBI): 80346
• RRID: AB_2880589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H6H4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	J Cell Biol; Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER; Authors - Shue Chen
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss
human	IHC	Aging (Albany NY); Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients; Authors - Shuping Luo
human	WB	J Cancer; REEP4 as Potential Biomarker Associated with Predictive Prognosis and Immune Response in Kidney Clear Cell Carcinoma; Authors - Zixuan Chen