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REDD1 Recombinant antibody, PBS Only

REDD1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L2

Cat no : 82650-1-PBS

Synonyms

DDIT4, 1L2, RTP801, REDD-1, HIF-1 responsive protein RTP801

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REDD1 Recombinant antibody (82650-1-RR)

    WB analysis using 82650-1-RR (same clone as 82650-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using REDD1 Recombinant antibody (82650-1-RR)

    WB analysis of HeLa using 82650-1-RR (same clone as 82650-1-PBS)

    Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82650-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82650-1-RR

    Affinity and Kinetic Characterization of 82650-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82650-1-RR against Human REDD1 were performed. The affinity constant is below 1 pM.

Product Information

82650-1-PBS targets REDD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen REDD1 fusion protein Ag0965
Full Name DNA-damage-inducible transcript 4
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC007714
Gene Symbol REDD1/DDIT4
Gene ID (NCBI) 54541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].