RECQL4 Polyclonal antibody

• Featured Product
• KD/KO Validated

RECQL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17008-1-AP

Synonyms

RecQ protein like 4, EC:5.6.2.4, DNA helicase, RecQ-like type 4, DNA 3'-5' helicase RecQ4, ATP-dependent DNA helicase Q4

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Validation Data Gallery

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  WB analysis using 17008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17008-1-AP

  IP result of anti-RECQL4 (IP:17008-1-AP, 4ug; Detection:17008-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17008-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RECQL4 antibody (17008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17008-1-AP targets RECQL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RECQL4 fusion protein Ag10707
• Full Name: RecQ protein-like 4
• Calculated Molecular Weight: 1208 aa, 133 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC013277
• Gene Symbol: RECQL4
• Gene ID (NCBI): 9401
• RRID: AB_2238324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IHC	Cancer Biol Med; RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.; Authors - Guosheng Lyu ; KD Validated
human	WB	FASEB J; UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.; Authors - Qiuling Huang
human	WB	J Biol Chem; The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.; Authors - Jiewei Wang
human	IF	Sci Rep; PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.; Authors - Sebastian Veith
human	IF	bioRxiv; USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication; Authors - Hannah L Mackay