RECQL Recombinant antibody

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  IF Staining of U-251 using 85024-6-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RECQL antibody (85024-6-RR, Clone: 242577F1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RECQL {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 85024-6-RR

Clone No.242577F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

DNA helicase, RecQ like type 1, DNA dependent ATPase Q1, DNA 3'-5' helicase Q1, ATP-dependent DNA helicase Q1, ATP dependent DNA helicase Q1

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Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85024-6-RR targets RECQL in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RECQL fusion protein Ag6834
• Full Name: RecQ protein-like (DNA helicase Q1-like)
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: BC001052
• Gene Symbol: RECQL
• Gene ID (NCBI): 5965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46063
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420)