RDX Polyclonal antibody

RDX Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26105-1-AP

Synonyms

DFNB24, radixin, RDX

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Validation Data Gallery

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  WB analysis using 26105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26105-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26105-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26105-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 26105-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26105-1-AP (RDX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26105-1-AP targets RDX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RDX fusion protein Ag23532
• Full Name: radixin
• Calculated Molecular Weight: 583 aa, 68 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC047109
• Gene Symbol: RDX
• Gene ID (NCBI): 5962
• RRID: AB_2880380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.