RCOR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RCOR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27686-1-AP

Synonyms

RCOR 1, RCOR, COREST

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Validation Data Gallery

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  WB analysis of C2C12 using 27686-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 27686-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27686-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27686-1-AP (RCOR1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cell, PC-12 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

27686-1-AP targets RCOR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCOR1 fusion protein Ag26770
• Full Name: REST corepressor 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: NM_015156
• Gene Symbol: RCOR1
• Gene ID (NCBI): 23186
• RRID: AB_2880947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RCOR1, also named as REST corepressor 1, is a 485 amino acid protein, which belongs to the CoREST family. RCOR1 is a essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. The calculated molecular weight of RCOR1 is a 53 kDa, but the phosphorylated RCOR1 is about 60-65 kDa.

Publications

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
rat	WB	Biochem Biophys Res Commun; Increased NRSF/REST in anterior cingulate cortex contributes to diabetes-related neuropathic pain.; Authors - Xu Xiao-Die
human	IHC	Ann Diagn Pathol; microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia.; Authors - Esmerina Tili
human	WB	Nat Commun; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition; Authors - Cheng Zeng ; KO Validated
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao
human	WB	Cell Rep; E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC; Authors - Feiyu Tang