RCN1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83638-3-RR (same clone as 83638-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00637-3

  Cytometric bead array standard curve of MP00637-3, RCN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83638-4-PBS. Detection antibody: 83638-3-PBS. Standard: Ag13256. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83638-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83638-3-PBS against Human RCN1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83638-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

RCN1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83638-3-PBS

Clone No.240473E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20

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Product Information

83638-3-PBS targets RCN1 as part of a matched antibody pair:

MP00637-3: 83638-4-PBS capture and 83638-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RCN1 fusion protein Ag13256
• Full Name: reticulocalbin 1, EF-hand calcium binding domain
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC010120
• Gene Symbol: RCN1
• Gene ID (NCBI): 5954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15293
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).