RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22335-1-AP

Synonyms

RCC1 I, CHC1

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Validation Data Gallery

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  WB analysis using 22335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22335-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22335-1-AP

  IP result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.

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  IHC staining of mouse testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 22335-1-AP

  Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HepG2 cells, mouse colon tissue, rat colon tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human small intestine tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

22335-1-AP targets RCC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCC1 fusion protein Ag17809
• Full Name: regulator of chromosome condensation 1
• Calculated Molecular Weight: 421 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC010067
• Gene Symbol: RCC1
• Gene ID (NCBI): 1104
• RRID: AB_10896498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.

Publications

Species	Application	Title
human	IHC	Pathol Oncol Res; RCC1 Expression as a Prognostic Marker in Colorectal Liver Oligometastases.; Authors - Yuxiang Deng