RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 22142-1-AP

Synonyms

RCC1 I, CHC1

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Validation Data Gallery

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  WB analysis using 22142-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22142-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22142-1-AP

  IP result of anti-RCC1 (IP:22142-1-AP, 4ug; Detection:22142-1-AP 1:8000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22142-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 22142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 22142-1-AP

  Immunofluorescent analysis of A431 cells using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, SH-SY5Y cells, COS-7 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22142-1-AP targets RCC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCC1 fusion protein Ag17569
• Full Name: regulator of chromosome condensation 1
• Calculated Molecular Weight: 421 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC010067
• Gene Symbol: RCC1
• Gene ID (NCBI): 1104
• RRID: AB_11183045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Publications

Species	Application	Title
human	IF	Curr Biol; Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.; Authors - Diana Vargas-Hurtado
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	IHC	Cell Death Dis; Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.; Authors - Lijun Qiao
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	J Cell Mol Med; SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing; Authors - Xi Chen