RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 22142-1-AP

Synonyms

RCC1 I, CHC1



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, SH-SY5Y cells, COS-7 cells, HeLa cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22142-1-AP targets RCC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17569
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_11183045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 22142-1-APDownload protocol
IHC protocol for RCC1 antibody 22142-1-APDownload protocol
IF protocol for RCC1 antibody 22142-1-APDownload protocol
IP protocol for RCC1 antibody 22142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Curr Biol

Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.

Authors - Diana Vargas-Hurtado
mouseWB

Biomaterials

Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

Authors - Yudong Song
humanWB

J Med Chem

Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.

Authors - Brianna D Mackie
humanIHC

Cell Death Dis

Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.

Authors - Lijun Qiao
mouseWB

Exp Gerontol

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

Authors - Shama R Iyer
humanWB

J Cell Mol Med

SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing

Authors - Xi Chen