RCAN2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RCAN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12900-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12900-1-AP (same clone as 12900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12900-1-AP (same clone as 12900-1-PBS)

  IP result of anti-RCAN2 (IP:12900-1-AP, 4ug; Detection:12900-1-AP 1:300) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12900-1-AP (same clone as 12900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 12900-1-AP (same clone as 12900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12900-1-PBS in a different storage buffer formulation.

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Product Information

12900-1-PBS targets RCAN2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCAN2 fusion protein Ag3597
• Full Name: regulator of calcineurin 2
• Calculated Molecular Weight: 225 aa, 25 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC038509
• Gene Symbol: RCAN2
• Gene ID (NCBI): 10231
• RRID: AB_10638913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RCAN2 is a member of the regulator of calcineurin (RCAN) protein family. These proteins play a role in many physiological processes by binding to the catalytic domain of calcineurin A, inhibiting calcineurin-mediated nuclear translocation of the transcription factor NFATC1. Expression of this gene in skin fibroblasts is upregulated by thyroid hormone, and the encoded protein may also play a role in endothelial cell function and angiogenesis.