RC3H1 Recombinant antibody

• 

  WB analysis using 84323-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84323-1-RR (RC3H1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

RC3H1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84323-1-RR

Clone No.241046G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84323-1-RR targets RC3H1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ring finger and CCCH-type zinc finger domains 1
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_172071
• Gene Symbol: RC3H1
• Gene ID (NCBI): 149041
• RRID: AB_3671865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5TC82
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)