RBPMS Polyclonal antibody

• Featured Product
• KD/KO Validated

RBPMS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 15187-1-AP

Synonyms

RNA-binding protein with multiple splicing, RBP-MS, RBP MS, Hermes

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Validation Data Gallery

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  WB analysis using 15187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15187-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15187-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15187-1-AP

  IP result of anti-RBPMS (IP:15187-1-AP, 4ug; Detection:15187-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 15187-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15187-1-AP (RBPMS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 15187-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15187-1-AP (RBPMS antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15187-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (15187-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 15187-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBPMS (15187-1-AP) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 19 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

15187-1-AP targets RBPMS in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBPMS fusion protein Ag7256
• Full Name: RNA binding protein with multiple splicing
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC003608
• Gene Symbol: RBPMS
• Gene ID (NCBI): 11030
• RRID: AB_2238431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.

Publications

Species	Application	Title
mouse	IF	Cell; Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact; Authors - Kenichi Toma
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
human	WB,IP	Cell Rep; Pan-cancer tRNA-derived fragment CAT1 coordinates RBPMS to stabilize NOTCH2 mRNA to promote tumorigenesis; Authors - Mengqian Yu
mouse	IF	Cell Rep; Comparative transcriptomic profiling reveals a role for Olig1 in promoting axon regeneration; Authors - Xiu-Qing Fu
mouse	IHC	Cell Rep; Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.; Authors - Thanh Hoang
mouse	IF	Sci Rep; Develop an efficient and specific AAV-based labeling system for Muller glia in mice; Authors - Yanxia Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (07-26-2022)

This antibody works great for western blot. I had a very specific band at the expected MW of RBPMS. Highly recommended!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000