RBPJ Polyclonal antibody

• Featured Product
• KD/KO Validated

RBPJ Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14613-1-AP

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

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Validation Data Gallery

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  WB analysis of mouse kidney using 14613-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14613-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14613-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14613-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14613-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14613-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14613-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14613-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14613-1-AP

  IP result of anti-RBPJ (IP:14613-1-AP, 3ug; Detection:14613-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

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  IHC staining of human pancreas cancer using 14613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14613-1-AP (RBPJ antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14613-1-AP (RBPJ antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human heart tissue, human liver tissue, MCF-7 cells, mouse pancreas tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

14613-1-AP targets RBPJ in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBPJ fusion protein Ag6206
• Full Name: recombination signal binding protein for immunoglobulin kappa J region
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC064976
• Gene Symbol: RBPJ
• Gene ID (NCBI): 3516
• RRID: AB_2253744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

Publications

Species	Application	Title
mouse	IHC	Cell Biosci; Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis.; Authors - Jiachen Lv
human	WB	J Biol Chem; RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal.; Authors - Fu YangXin Y ; KD Validated
mouse	WB	Front Pharmacol; DAPT Attenuates Cadmium-Induced Toxicity in Mice by Inhibiting Inflammation and the Notch/HES-1 Signaling Axis.; Authors - Jia-Ying Yang
mouse	WB	Inflamm Res; Salidroside alleviates cadmium-induced toxicity in mice by restoring the notch/HES-1 and RIP1-driven inflammatory signaling axis.; Authors - Xing-Mei Shu
mouse	WB	Inflamm Res; Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation; Authors - Teng‑Teng Ren
mouse	WB	Neurotoxicology; Edaravone attenuates cadmium-induced toxicity by inhibiting oxidative stress and inflammation in ICR mice.; Authors - Sheng-Rui Fan