RBP4 Polyclonal antibody

RBP4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29550-1-AP

Synonyms

Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein

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Validation Data Gallery

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  WB analysis using 29550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29550-1-AP (RBP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29550-1-AP targets RBP4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: retinol binding protein 4, plasma
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC020633
• Gene Symbol: RBP4
• Gene ID (NCBI): 5950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBP4 (Retinol-binding protein 4), also known as PRO2222. It is expected to be located in the cytoplasm, which is expressed in renal tubules, pancreatic islets and hepatocytes with positivity in plasma. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post- translationally and results in defective delivery and supply to the epidermal cells. The molecular weight of RBP4 is 23 kDa.