RBM7 Polyclonal antibody

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (3)

Cat no : 21896-1-AP

Synonyms

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

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Validation Data Gallery

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  WB analysis of HepG2 using 21896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21896-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21896-1-AP (RBM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

21896-1-AP targets RBM7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM7 fusion protein Ag11106
• Full Name: RNA binding motif protein 7
• Calculated Molecular Weight: 266 aa, 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC034381
• Gene Symbol: RBM7
• Gene ID (NCBI): 10179
• RRID: AB_2878938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Publications

Species	Application	Title
		Nat Commun; Temporal-iCLIP captures co-transcriptional RNA-protein interactions; Authors - Ross A Cordiner
human	WB	Mol Cell; ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts; Authors - Jérôme O Rouvière
human	WB	Mol Cell; P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.; Authors - Andrii Bugai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody that detect Rbm7 in U2OS cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

RBM7 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:21896-1-AP)

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect RBM7 with Western blot in HEK293T WT cell lysate, which worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

RBM7 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:21896-1-AP)

FH

Roy (Verified Customer) (06-15-2024)

Works well to detect the NEXT complex subunit RBM7 from HeLa extracts (1/1000 Dilution, overnight Incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa