RBM41 Polyclonal antibody

RBM41 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15784-1-AP

Synonyms

bB383K5.1, RBM41, RNA binding motif protein 41, RNA binding protein 41

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Validation Data Gallery

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  WB analysis using 15784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15784-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15784-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15784-1-AP (RBM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 15784-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human endometrial cancer using 15784-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15784-1-AP (RBM41 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15784-1-AP

  Immunofluorescent analysis of HepG2 cells, using RBM41 antibody 15784-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, SGC-7901 cells, HepG2 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15784-1-AP targets RBM41 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM41 fusion protein Ag8483
• Full Name: RNA binding motif protein 41
• Calculated Molecular Weight: 413 aa, 47 kDa
• Observed Molecular Weight: 43-47 kDa
• GenBank Accession Number: BC006986
• Gene Symbol: RBM41
• Gene ID (NCBI): 55285
• RRID: AB_2176112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.