RBM39 Monoclonal antibody, PBS Only

RBM39 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2C8

Cat no : 67420-1-PBS

Synonyms

CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

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Validation Data Gallery

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  WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67420-1-Ig (RBM39 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM39 antibody (67420-1-Ig, Clone: 2D2C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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Product Information

67420-1-PBS targets RBM39 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM39 fusion protein Ag15766
• Full Name: RNA binding motif protein 39
• Calculated Molecular Weight: 530 aa, 59 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC141835
• Gene Symbol: RBM39
• Gene ID (NCBI): 9584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.