RBM39 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21339-1-AP

Synonyms

RNA binding protein 39, HCC1, fSAP59, CC1.3, CAPERalpha

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Validation Data Gallery

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  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21339-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21339-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 21339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21339-1-AP (RBM39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human skeletal muscle tissue, mouse kidney tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Product Information

21339-1-AP targets RBM39 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM39 fusion protein Ag15584
• Full Name: RNA binding motif protein 39
• Calculated Molecular Weight: 530 aa, 59 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC141835
• Gene Symbol: RBM39
• Gene ID (NCBI): 9584
• RRID: AB_10733352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	J Proteome Res; Proximity Labeling, Quantitative Proteomics, and Biochemical Studies Revealed the Molecular Mechanism for the Inhibitory Effect of Indisulam on the Proliferation of Gastric Cancer Cells.; Authors - Jiaqi Lu ; KD Validated
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting
human	IHC,RIP	Cell Death Discov; The cancer-testis lncRNA LINC01977 promotes HCC progression by interacting with RBM39 to prevent Notch2 ubiquitination; Authors - Anliang Xia
human	WB	Mol Cell Biochem; LncRNA MCM3AP-AS1 promotes chemoresistance in triple-negative breast cancer through the miR-524-5p/RBM39 axis; Authors - Yueping Wang