Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 16823-1-AP
IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.
Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 16823-1-AP
Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:12000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 16823-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 16823-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HEK-293 using 16823-1-AP
IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.
IF/ICC Figures
IF Staining of HepG2 using 16823-1-AP
Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RBM26 Polyclonal antibody
Catalog Number
16823-1-AP
Citations
1
Dilutions
WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
