RBM25 Recombinant antibody

• 

  WB analysis using 84968-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84968-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

RBM25 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84968-4-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

RED120, RBM 25, Protein S164, NET52, Arg/Glu/Asp-rich protein of 120 kDa

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Add to Basket

Please visit your regions distributor:

---

Proteintech Guarantee

---

---

Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells，Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84968-4-RR targets RBM25 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBM25 fusion protein Ag18244
• Full Name: RNA binding motif protein 25
• Calculated Molecular Weight: 843 aa, 100 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC113389
• Gene Symbol: RBM25
• Gene ID (NCBI): 58517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P49756
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.