RBM12B Polyclonal antibody

RBM12B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17137-1-AP

Synonyms

RBM12B, RNA binding motif protein 12B, RNA binding protein 12B

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Validation Data Gallery

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  WB analysis using 17137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17137-1-AP (RBM12B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17137-1-AP (RBM12B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17137-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM12B antibody (17137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17137-1-AP targets RBM12B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM12B fusion protein Ag10894
• Full Name: RNA binding motif protein 12B
• Calculated Molecular Weight: 765 aa, 87 kDa, 118 kDa
• Observed Molecular Weight: 118-130 kDa
• GenBank Accession Number: BC039260
• Gene Symbol: RBM12B
• Gene ID (NCBI): 389677
• RRID: AB_2175746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An