RBCK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBCK1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 26367-1-AP

Synonyms

C20orf18, HBV associated factor 4, HOIL 1, HOIL1, RBCK1, RBCK2, RING finger protein 54, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4

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Validation Data Gallery

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  IP experiment of human placenta using 26367-1-AP

  IP result of anti-RBCK1 (IP:26367-1-AP, 4ug; Detection:26367-1-AP 1:300) with human placenta tissue lysate 1520ug.

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  IHC staining of human placenta using 26367-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26367-1-AP (RBCK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26367-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26367-1-AP (RBCK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

The immunogen of 26367-1-AP is RBCK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBCK1 fusion protein Ag24405
• Full Name: RanBP-type and C3HC4-type zinc finger containing 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC015219
• Gene Symbol: RBCK1
• Gene ID (NCBI): 10616
• RRID: AB_2880493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Cell Death Dis; RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling; Authors - Zhiguo Niu
human	WB,IHC	Oncogene; The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis; Authors - Yapeng Wang ; KD Validated
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu
human	IHC	Hum Cell; Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1.; Authors - Guanghui Wang ; KD Validated
human	WB	BMC Musculoskelet Disord; Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.; Authors - Lin Chen
human	WB	Cell Death Dis; RBCK1 promotes p53 degradation via ubiquitination in renal cell carcinoma.; Authors - Sifan Yu ; KD Validated