RBBP6 Polyclonal antibody

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• KD/KO Validated

RBBP6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11882-1-AP

Synonyms

MY038, P2P R, P2PR, PACT, Protein P2P R, RBBP6, RBQ 1, RBQ1, SNAMA

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Validation Data Gallery

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  WB analysis using 11882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11882-1-AP targets RBBP6 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBBP6 fusion protein Ag2484
• Full Name: retinoblastoma binding protein 6
• Calculated Molecular Weight: 1792 aa, 200 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC029352
• Gene Symbol: RBBP6
• Gene ID (NCBI): 5930
• RRID: AB_2177794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

Publications

Species	Application	Title
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated