RBBP5 Polyclonal antibody

RBBP5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13181-1-AP

Synonyms

RBBP 5, RBBP5, RBQ3, SWD1

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Validation Data Gallery

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 13181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13181-1-AP targets RBBP5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBBP5 fusion protein Ag3848
• Full Name: retinoblastoma binding protein 5
• Calculated Molecular Weight: 538 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC037284
• Gene Symbol: RBBP5
• Gene ID (NCBI): 5929
• RRID: AB_2177773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR.

Publications

Species	Application	Title
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu