RB1CC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RB1CC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21269-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Validation Data Gallery

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  WB analysis of HEK-293 using 21269-1-AP

  WB result of RB1CC1 antibody (21269-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

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  WB analysis of HeLa using 21269-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21269-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21269-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21269-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21269-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21269-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 21269-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21269-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21269-1-AP targets RB1CC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RB1CC1 fusion protein Ag15739
• Full Name: RB1-inducible coiled-coil 1
• Calculated Molecular Weight: 1594 aa, 183 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC017556
• Gene Symbol: RB1CC1
• Gene ID (NCBI): 9821
• RRID: AB_10733100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RB1CC1.