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  • KD/KO Validated

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17250-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17250-1-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1CC1 fusion protein Ag10508
Full Name RB1-inducible coiled-coil 1
Calculated Molecular Weight 1594 aa, 183 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC017556
Gene Symbol RB1CC1
Gene ID (NCBI) 9821
RRIDAB_10666428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 17250-1-APDownload protocol
IHC protocol for RB1CC1 antibody 17250-1-APDownload protocol
IF protocol for RB1CC1 antibody 17250-1-APDownload protocol
IP protocol for RB1CC1 antibody 17250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Authors - Suresh Kumar
humanIF

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai
humanWB,IF

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
humanWB

Nat Cell Biol

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.

Authors - Andrew M Leidal
  • KO Validated
humanWB,IHC

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human endometrial stromal cells