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RB1CC1 Polyclonal antibody
RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17250-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | mouse brain tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 18 publications below |
WB | See 69 publications below |
IHC | See 5 publications below |
IF | See 32 publications below |
IP | See 2 publications below |
CoIP | See 3 publications below |
Product Information
17250-1-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RB1CC1 fusion protein Ag10508 |
Full Name | RB1-inducible coiled-coil 1 |
Calculated Molecular Weight | 1594 aa, 183 kDa |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | BC017556 |
Gene Symbol | RB1CC1 |
Gene ID (NCBI) | 9821 |
RRID | AB_10666428 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
IHC protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
IF protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
IP protocol for RB1CC1 antibody 17250-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. | ||
Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
| ||
Nat Cell Biol The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.
| ||
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SANGAPPA (Verified Customer) (01-04-2020) | Works very good in western blotting!
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