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RB1CC1 Polyclonal antibody
RB1CC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17250-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells, Juekat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17250-1-AP targets RB1CC1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RB1CC1 fusion protein Ag10508|
|Full Name||RB1-inducible coiled-coil 1|
|Calculated molecular weight||1594 aa, 183 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||BC017556|
|Gene ID (NCBI)||9821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.
|Product Specific Protocols|
|WB protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IHC protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IF protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|IP protocol for RB1CC1 antibody 17250-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.
A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
Nat Cell Biol
The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.
TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SANGAPPA (Verified Customer) (01-04-2020)
Works very good in western blotting!