RB1CC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

101

Reviews (1)

Cat no : 17250-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Validation Data Gallery

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  WB analysis of HEK-293 using 17250-1-AP

  WB result of RB1CC1 antibody (17250-1-AP, 1:2000) with si-Control and si-RB1CC1 transfected HEK293 cells.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17250-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 2500ug.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 1600ug.

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17250-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RB1CC1 antibody (17250-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 19 publications below
• WB: See 71 publications below
• IHC: See 5 publications below
• IF: See 32 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

17250-1-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RB1CC1 fusion protein Ag10508
• Full Name: RB1-inducible coiled-coil 1
• Calculated Molecular Weight: 1594 aa, 183 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC017556
• Gene Symbol: RB1CC1
• Gene ID (NCBI): 9821
• RRID: AB_10666428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Publications

Species	Application	Title
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai
human	WB,IF	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human	WB	Nat Cell Biol; The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.; Authors - Andrew M Leidal ; KO Validated
human	WB,IHC	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human endometrial stromal cells

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