RB1CC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10069-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Validation Data Gallery

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  WB analysis of HEK-293 using 10069-1-AP

  WB result of RB1CC1 antibody (10069-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

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  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10069-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10069-1-AP (RB1CC1 Antibody) at dilution of 1:50.

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  IHC staining of human appendicitis using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10069-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (10069-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, mouse skeletal muscle tissue
• Positive IHC detected in: human appendicitis tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

10069-1-AP targets RB1CC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RB1CC1 fusion protein Ag0003
• Full Name: RB1-inducible coiled-coil 1
• Calculated Molecular Weight: 1594 aa, 183 kDa
• Observed Molecular Weight: 183 kDa
• GenBank Accession Number: BC017556
• Gene Symbol: RB1CC1
• Gene ID (NCBI): 9821
• RRID: AB_2877722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; 26S proteasomes become stably activated upon heat shock when ubiquitination and protein degradation increase.; Authors - Donghoon Lee
human	IF	Dev Cell; The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.; Authors - Claudia Puri